Fig. 3.

OdDHL inhibits proliferation of naïve CD4⁺ T cells. CD44⁻ CD25^lo cells (5 × 10⁵) were grown on precoated anti-CD3/anti-CD28 plates under conditions conducive for Th1 (i), Th2 (ii), Th17 (iii), and Treg (iv) cell polarization. Cells were grown in the presence or absence of 10 μM OdDHL for 48 h, and proliferation was assessed using [³H]thymidine incorporation (a) or CFSE dilution (c). Two control conditions were also included, medium alone and PBS containing 0.01% DMSO. A representative FACS plot showing a dose-dependent inhibitory effect of OdDHL up to 10 μM (P = 0.006) on Th1 polarization is shown (b). Data are representative of three independent experiments. Statistical analysis was carried out by using the Mann-Whitney U test in GraphPad Prism.