Fig. 4.

OdDHL inhibits CD4⁺ naïve T cell polarization in vitro. Naïve splenic T cells (5 × 10⁵) were cultured for 4 days on anti-CD3/anti-CD28-precoated plates. Cells were grown under polarizing conditions specific to the T cell subset required. Representative FACS plots are shown of control cells (a) and of cells grown in the presence of 10 μM OdDHL (b) under Th1-polarizing conditions. A summary of between three and five independent experiments is shown where control and OdDHL-treated cells were grown under polarizing conditions for Th1 (c), Th2 (d), Th17 (e), and Treg (f) cells. Statistical analysis was carried out by using a Mann-Whitney U test in GraphPad Prism. Mean values are indicated by a horizontal bar. Ax488, Alexa Fluor 488.