Fig. 5.

(a) OdDHL shows minimal inhibitory effect on the proliferation of polarized CD4⁺ T cells. Rested polarized T cells (5 × 10⁵) were restimulated by plating in anti-CD3/anti-CD28-precoated plates under conditions essential for the maintenance of each T cell subset: Th1 (i), Th2 (ii), Th17 (iii), and Treg (iv). Proliferation was assessed using [³H]thymidine incorporation and CSFE dilution. Two control conditions were also included, medium alone and PBS containing 0.01% DMSO. A summary of five independent experiments is shown for [³H]thymidine (a) and CFSE dilution (b). Statistical analysis was carried out by using a Mann-Whitney U test in GraphPad Prism.