Fig. 6.
OdDHL shows minimal inhibitory effect on polarized CD4+ T cells in vitro. Rested polarized T cells (5 × 105) were cultured for 4 days on anti-CD3/anti-CD28-precoated plates under conditions essential for the maintenance of each T cell subset. Representative FACS plots are shown of Th1 cells grown in medium (a) or 10 μM OdDHL (b). A summary of between three and five independent experiments showing the effect of OdDHL on polarized cells subsets is also shown for Th1 (c), Th2 (d), Th17 (d), and Treg (f) cells. Statistical analysis was carried out by using a Mann-Whitney U test in GraphPad Prism.