Table 4.
Sensitivity of antigen capture ELISA constructed from various GXM MAbs for detection of GXM of the four major serotypes
| MAb used for construction of ELISAa | Minimum concn of GXM (ng/ml) producing a positive resultb |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| A |
B |
C |
D |
||||||
| CN6 | MU-1 | 184 | 409 | 34 | 298 | 24066 | 127 | M0024 | |
| F12D2 | 0.40 | 0.41 | 0.41 | 0.47 | 6.8 | 2.1 | 2.3 | 1.9 | 2.9 |
| 3C2 | 0.77 | 0.61 | 0.67 | 0.77 | 26 | 5.2 | 6.7 | 2.5 | 2.8 |
| 471 | 0.58 | 0.45 | 0.60 | 0.56 | 360 | 30 | 37 | 4.3 | 2.3 |
| 339 | 0.82 | 0.90 | 8.1 | 7.5 | >2,000 | 1,200 | >2,000 | 1.1 | 0.56 |
| 1255 | 1.9 | 1.3 | 6.7 | 6.2 | 1,100 | 40 | 110 | 1.4 | 0.80 |
a
The indicated MAb was used for the capture phase of the ELISA, and a HRPO conjugate of the same MAb was used in the detection phase.
b
Results are shown for GXM isolated from different strains of each serotype. >2,000, GXM of some strains of serotype C at concentrations of 2,000 ng/ml failed to produce an OD of 0.5.