Table 5.
Optimization of ELISA performance using combinations of MAbs F12D2 and 339
| MAbs used for ELISA constructiona |
Minimum concn of GXM (ng/ml) producing a positive resultb |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| A |
B |
C |
D |
|||||||
| Capture MAb(s) | Indicator MAb(s) | CN6 | MU-1 | 184 | 409 | 34 | 298 | 24066 | 127 | M0024 |
| F12D2 | F12D2 | 0.47 | 0.29 | 0.78 | 0.80 | 6.8 | 2.1 | 2.3 | 2.8 | 3.2 |
| F12D2 | 339 | 1.1 | 0.70 | 3.7 | 5.0 | 1,700 | 220 | >2,000 | 0.93 | 1.1 |
| F12D2 | F12D2/339 | 0.64 | 0.43 | 1.1 | 1.2 | 9.5 | 2.8 | 2.8 | 0.91 | 1.0 |
| 339 | F12D2 | 0.82 | 0.51 | 0.94 | 1.1 | 2,000 | 98 | 1,500 | 2.7 | 2.4 |
| 339 | 339 | 0.83 | 1.0 | 9.7 | 11 | >2,000 | 1,100 | >2,000 | 1.0 | 0.65 |
| 339 | F12D2/339 | 1.0 | 0.62 | 2.0 | 2.0 | >2,000 | 155 | 1,900 | 0.97 | 0.61 |
| F12D2/339 | F12D2 | 0.46 | 0.27 | 0.74 | 0.75 | 6.6 | 1.9 | 2.0 | 2.4 | 2.8 |
| F12D2/339 | 339 | 1.2 | 0.71 | 3.6 | 4.4 | 2,000 | 420 | >2,000 | 0.67 | 0.51 |
| F12D2/339 | F12D2/339 | 0.76 | 0.44 | 0.85 | 0.86 | 10 | 2.7 | 2.8 | 0.63 | 0.51 |
a
The indicated MAb or a 50:50 mixture of the two MAbs was used in either the capture phase of the ELISA or as a HRPO conjugate (indicator MAb[s]).
b
Results are shown for GXM isolated from different strains of each serotype. >2,000, GXM of some strains of serotype C at concentrations of 2,000 ng/ml failed to produce an OD of 0.5.