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Recognition of L. intracellularis proteins by sera from infected pigs. Shown is a Western blot of L. intracellularis protein extract probed with sera pooled from 10 pigs naturally infected with L. intracellularis (lane A) and the corresponding SDS-PAGE gel (lane B). A protein of approximately 72 kDa was strongly recognized by sera, and the corresponding region of the SDS-PAGE gel was excised and analyzed by LC-ESI-MS/MS.
