Western blot analysis of B. mandrillaris protein extracts. (A to C) Western blot analysis of B. mandrillaris extracts using a hyperimmune rabbit serum (A), Balamuthia-infected patient serum S-1 (B), and a normal blood donor serum, S-BD-3 (C). Lanes: M, molecular mass markers (in kilodaltons); 1, E6 cells; 2B.m-2 (human isolate CDC:V416); 3 B.m-3 (horse isolate CDC:V433); 4, B.m-4 (mandrill isolate CDC:V039); 5, B.m-5 (human isolate CDC:V188); 6, B.m-8 (human isolate CDC:V194); 7, Acanthamoeba castellanii (human isolate CDC:V042); 8, Naegleria fowleri (human isolate CDC:V414).