Table 2.
Probes included in probe set 3
| Probe identifier | Taxonomic group(s) detected | Probe sequence | % False positive/% false negativea | Mean correct signala | Standard deviation correct signala |
|---|---|---|---|---|---|
| 1_1 | Bacteroides | TTGCGGCTCAACCGTAAAATTG | 0/0 | 1,723.54 | 245.51 |
| 1_1_3 | Parabacteroides | CGCCTGCCTCAAACATA | 0/0 | 733.62 | NA |
| 1_2_2 | Bacteroides (dorei, fragilis, thetaiotaomicron, vulgatus) | GCACTCAAGACATCCAGTATCAACTG | 0/0 | 1,261.71 | 435.04 |
| 1_3_3 | Bacteroides (dorei, fragilis, thetaiotaomicron, vulgatus) | AGGGCAGTCATCCTTCACG | 0/0 | 1,157.96 | 391.09 |
| 2_1_min1b | Gamma-proteobacteria | CAGGTGTAGCGGTGAAATGCGTAGAGAT | 14/0 | 1,711.24 | 201.24 |
| 2_1_1 | Haemophilus | ACGCTCGCACC | 0/0 | 270.16 | NA |
| 2_3_2 | Gamma-proteobacteria subgroup | CGGGGATTTCACATCTGA | 8/0 | 141.42 | NA |
| 2_4_1 | Gamma-proteobacteria subgroup | TGCCAGTTTCGAATGCAGTT | 4/0 | 1,677.81 | 251.28 |
| 2_5_1 | Gamma-proteobacteria subgroup | GTGCTTCTTCTGCGGGTAA | 0/0 | 611.51 | 155.12 |
| 2_7_1 | Salmonella | TGTTGTGGTTAATAACCGCAGCAATTGA | 4/0 | 1,527.71 | NA |
| 3_2 | Proteobacteria | ACGCTTGCACCCT | 5/0 | 809.64 | 278.90 |
| 4_1 | Firmicutes (Lactobacillales, Clostridium perfringens, Staphylococcus) | CGATCCGAAAACCTTCTTCACT | 6/0 | 1,799.51 | 538.14 |
| 4_2_3 | Lactobacillus subgroup | GCTACACATGGAGTTCCA | 29/0 | 278.64 | 14.67 |
| 4_3_1 | Clostridium ramosum | CCGTCACTCGGCTACCATTTC | 0/0 | 2,429.10 | NA |
| 4_4_2 | Enterococcus, Listeria | TCCAATGACCCTCCC | 0/0 | 640.06 | 125.05 |
| 4_5_2 | Streptococcus pyogenes | GATTTTCCACTCCCACCAT | 0/0 | 1,556.65 | NA |
| 4_6_1 | Streptococcus sanguinis | CACTCTCACACCCGTT | 0/0 | 978.28 | NA |
| 4_7_2 | Listeria | CCGTCAAGGGACAAG | 0/0 | 678.60 | NA |
| 4_8_1 | Streptococcus pneumoniae, Enterococcus | GTTGCTCGGTCAGACTT | 12/0 | 1,593.28 | NA |
| 5_1 | Firmicutes (Clostridia, Bacillales, Enterococcus, Lactobacillus) | GGACAACGCTTGCCAC | 6/0 | 1,315.09 | 417.36 |
| 5_1_2 | Staphylococcus | CGTGGCTTTCTGATTAGGTA | 0/0 | 654.06 | NA |
| 5_2_1 | Clostridium neonatale | CGTAGTTAGCCGTGG | 0/0 | 0.00 | 0.00 |
| 6_1_4 | Bifidobacterium longum | TGCTTATTCAACGGGTAAACT | 0/0 | 2,071.50 | 492.05 |
| 6_2 | Actinobacteria | CGTAGGCGGTTCGTCGCGT | 0/0 | 1,417.55 | 243.38 |
| 6_2_2 | Bifidobacterium breve | CGGTGCTTATTCGAAAGGTACACT | 0/0 | 1,928.16 | NA |
| UNI01 | 16S Universal | CGTATTACCGCGGCTGCTGGCA | NA | NA | NA |
| HYC01 | Hybridization control | GTAGCATTCGATTCGGGCAA | NA | NA | NA |
a
NA, not applicable because the probe has only one control target bacterium.