Table 2.
Median serum cytokine concentrations and comparisons of variability across labs and lots for Luminex and MSD analytes with a majority of detectable results
| Platform, manufacturer, and cytokine | Laba | Median concn (pg/ml)b |
P value |
|||
|---|---|---|---|---|---|---|
| Lot 1 | Lot 2 | Lab-lot interactionc | Lab effectd | Lot effecte | ||
| Luminex | ||||||
| Bio-Rad | ||||||
| IL-1β | A | <0.1 (<0.1–0.3) | 0.4 (0.3–0.8) | NE | ||
| B | <0.1 (<0.1–0.1) | 0.1 (<0.1–0.3) | 0.003 | NEf | ||
| IL-4 | A | <0.1 (<0.1–0.2) | 0.8 (0.6–0.9) | <0.001 | ||
| B | <0.1 (<0.1–<0.1) | 0.3 (0.2–0.6) | 0.22 | <0.001 | ||
| TNF-α | A | 0.1 (<0.1–5.5) | 1.4 (0.7–5.9) | <0.001 | ||
| B | 0.1 (<0.1–3.3) | 1.2 (0.7–4.7) | 0.09 | 0.11 | ||
| BioSource | ||||||
| IL-1β | A | 2.8 (1.5–6.1) | 2.0 (0.9–5.2) | 0.02 | ||
| C | 1.8 (0.9–5.4) | 1.3 (1.0–3.0) | 0.48 | 0.05 | ||
| IL-6 | A | 0.8 (<0.1–2.7) | 2.2 (0.2–4.6) | NE | ||
| C | 0.2 (0.2–1.4) | 0.5 (<0.1–1.4) | 0.01 | NE | ||
| IL-8 | A | 29 (19–100) | 26 (16–140) | 0.33 | ||
| C | 21 (11–109) | 26 (12–101) | 0.30 | 0.05 | ||
| IL-10 | A | 0.5 (<0.1–2.0) | 1.3 (1.0–2.6) | <0.001 | ||
| C | 0.3 (<0.1–1.6) | 1.7 (0.3–2.7) | 0.80 | 0.06 | ||
| Linco | ||||||
| IL-2 | A | <0.1 (<0.1–0.8) | 1.2 (0.4–2.9) | NE | ||
| B | <0.1 (<0.1–2.5) | 1.8 (0.4–3.8) | 0.37 | NE | ||
| C | 2.3 (1.7–3.2) | 0.7 (0.1–1.5) | <0.001 | NE | ||
| IL-5 | A | 0.1 (<0.1–0.5) | 0.1 (<0.1–0.7) | NE | ||
| B | 0.1 (<0.1–0.5) | 0.1 (<0.1–0.9) | 0.80 | NE | ||
| C | 0.7 (0.3–0.9) | 0.1 (0.1–0.6) | <0.001 | NE | ||
| IL-6 | A | 4.8 (1.9–14.6) | 3.9 (1.8–10.9) | 0.77 | ||
| B | 4.6 (1.8–13.4) | 5.0 (2.0–14.0) | 0.07 | 0.58 | ||
| C | 7.2 (3.0–13.0) | 4.6 (2.3–15.2) | 0.21 | 0.005 | ||
| IL-7 | A | 9.8 (5.4–15.9) | 7.3 (4.2–10.6) | NE | ||
| B | 7.4 (4.5–10.5) | 7.9 (4.2–9.6) | 0.04 | NE | ||
| C | 7.3 (4.2–12.8) | 8.3 (4.3–11.9) | 0.004 | NE | ||
| IL-8 | A | 15.5 (6.8–55.0) | 10.0 (4.7–28.5) | NE | ||
| B | 11.2 (5.9–30.2) | 10.4 (5.6–28.6) | <0.001 | NE | ||
| C | 5.4 (3.8–23.9) | 9.7 (4.7–35.5) | <0.001 | NE | ||
| IL-10 | A | 12.0 (7.8–19.5) | 9.8 (6.3–16.2) | NE | ||
| B | 8.9 (6.2–15.9) | 11.0 (7.1–20.9) | 0.03 | NE | ||
| C | 7.4 (5.0–11.7) | 8.2 (3.5–14.8) | 0.68 | NE | ||
| IL-13 | A | 3.5 (0.2–39.7) | 2.1 (0.2–24.0) | 0.09 | ||
| B | 1.5 (<0.1–21.2) | 0.9 (<0.1–30.5) | 0.35 | 0.003 | ||
| C | 7.8 (0.7–50.1) | 6.0 (1.0–37.4) | 0.47 | 0.002 | ||
| GM-CSF | A | <0.1 (<0.1–3.2) | <0.1 (<0.1–1.2) | NE | ||
| B | 0.5 (<0.1–2.3) | 0.2 (0.1–2.5) | 0.20 | NE | ||
| C | 2.2 (0.8–3.5) | <0.1 (<0.1–0.5) | <0.001 | NE | ||
| TNF-α | A | 8.8 (5.5–15.9) | 7.2 (5.1–11.7) | NE | ||
| B | 6.6 (4.6–12.3) | 8.0 (6.3–14.6) | <0.001 | NE | ||
| C | 6.3 (5.0–9.2) | 6.7 (4.3–12.5) | <0.001 | NE | ||
| MSD | ||||||
| IL-2 | E | 2.5 (0.7–5.2) | 0.8 (0.8–2.5) | 0.007 | ||
| F | 0.4 (0.4–2.2) | NIg | <0.001 | |||
| IL-5 | E | 0.9 (0.7–1.6) | 1.3 (1.0–2.0) | 0.01 | ||
| F | 0.5 (0.3–0.9) | NI | <0.001 | |||
| IL-6 | E | 3.7 (3.1–5.4) | 1.8 (1.2–2.3) | NE | <0.001 | |
| IL-8 | E | 19 (12–83) | 21 (14–75) | 0.14 | ||
| F | 13 (7.7–59) | NI | <0.001 | |||
| IL-10 | E | 5.1 (4.0–7.8) | 5.6 (3.5–9.8) | 0.45 | ||
| F | 2.3 (1.4–4.2) | NI | <0.001 | |||
| IL-12 p70 | E | 4.8 (3.3–10.2) | 4.7 (1.4–9.8) | 0.26 | ||
| F | 2.7 (1.0–5.7) | NI | <0.001 | |||
| IL-13 | E | 4.7 (2.7–9.0) | 6.7 (4.8–10.7) | |||
| F | 1.6 (0.7-3.7) | NI | <0.001 | |||
| TNF-α | E | 15.0 (9.7–22.1) | 15.9 (10.6–22.2) | 0.52 | ||
| F | 10.2 (7.4–19.5) | NI | <0.001 | |||
Bio-Rad data available only from labs A and B; BioSource data available only from labs A and C.
Data in parentheses are interquartile ranges (25th to 75th percentiles).
For Luminex, P values are from multivariate model for pairwise lab-lot interaction using lab A as the reference; all comparisons with P values of <0.05 are shown in boldface.
For Luminex, P values are from multivariate model for pairwise lab effect only (regardless of lot), using lab A as the reference. For MSD, P values are from multivariate model for lab effects in lot 1 using lab E as the reference, except for IL-6, which was not evaluable since it was performed as a separate singleplex assay only in lab E.
For Luminex, P values are from multivariate model for lot effect only (regardless of lab). For MSD, P values are from multivariate model for lot effects in lab E.
NE, not evaluable due to significant lab-lot interaction.
NI, lot 2 was not included in lab F assays, so no lab-lot interaction analyses were possible.