Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source |
|---|---|---|
| B. cereus | ||
| ATCC 14579 | Wild-type strain | |
| ATCC 14579 ΔbclA | Kanr; bclA::kan deletion | This study |
| ATCC 14579 ΔexsH | Tetr; exsH::tet deletion | This study |
| ATCC 14579 ΔbclA ΔexsH | Kanr Tetr; bclA::kan and exsH::tet deletions | This study |
| E. coli K-12 | ||
| TG1 | Δ(lac-proAB) supE thi hsdΔ5 (F′ traD36 proA+proB+lacIqlacZΔM15) | |
| ET12567 | F′ dam-13::Tn9 dcm-6 hsdM hsdR recF143 zjj-202::Tn10 glaK2 galT22 ara14 pacY1 xyl-5 leuB6 thi-1 | |
| Plasmids | ||
| pHT304 | Ampr Eryr; shuttle vector for E. coli and B. cereus | 1 |
| pRN5101 | Thermosensitive vector | 20 |
| pDG786 | Source of the Kanr resistance cassette | 17 |
| pHTS2 | Source of the Tetr resistance cassette | 30 |
| pRN5101 ΔbclA ATCC 14579 Kanr | Kanr; plasmid used to delete bclA | This study |
| pRN5101 ΔexsH ATCC 14579 Tetr | Tetr; plasmid used to delete exsH | This study |
| pHT304 bclA ATCC 14579 | Ampr Eryr; plasmids containing the bclA gene and 317 bp upstream and 138 bp downstream; used for complementation | This study |
| pHT304 exsH ATCC 14579 | Ampr Eryr; plasmids containing the exsH gene and 465 bp upstream and 914 bp downstream; used for complementation | This study |
| pYL304 | Ampr Eryr,; plasmids expressing a BclA protein of 189 aa with the CTD deleted; bclA-ΔCT | This study |
| pYL306 | Ampr Eryr; plasmid expressing a BclA protein of 45 aa with the CLR and CTD deleted; bclA-ΔCTCLR | This study |
a
Kanr, kanamycin resistance; Ampr, ampicillin resistance; Tetr, tetracycline resistance; Eryr, erythromycin resistance.