Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source |
|---|---|---|
| E. coli strains | ||
| BW25113 | K-12; λ−; F−lacIqrrnBT14 ΔlacZWJ16hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | 12 |
| JW0855 | BW25113, poxB::Kanr | 6 |
| JW2294 | BW25113, pta::Kanr | 6 |
| JW1841 | BW25113, zwf::Kanr | 6 |
| JW3205 | BW25113, mdh::Kanr | 6 |
| JW1375 | BW25113, ldhA::Kanr | 6 |
| JW4115 | BW25113, frdA::Kanr | 6 |
| JW1095 | BW25113, ndh::Kanr | 6 |
| JW1228 | BW25113, adhE::Kanr | 6 |
| BFA7.001(λDE3) | BW25113, λDE3 Δzwf Δmdh ΔfrdA Δndh Δpta ΔpoxB ΔldhA::Kan | This study |
| BFA8.001(λDE3) | BW25113, λDE3 Δzwf Δmdh ΔfrdA Δndh Δpta ΔpoxB ΔldhA ΔadhE::Kan | This study |
| BL21(DE3) | F−ompT hsdSB(rB− mB−) gal dcm (λDE3) | Invitrogen (catalog no. C600003) |
| Plasmids | ||
| pSA55 | ColE1 ori, plac::kivd::adh2; Ampr | 3 |
| pSA69 | ColE1 ori, plac::alsS::ilvC::ilvD; Kanr | 3 |
| pCOLADuet | ColA ori; Kanr | Novagen (catalog no. 71406-3) |
| pET24b | f1 ori; Kanr | Novagen (catalog no. 69750-3) |
| pCT01 | pCOLA pT7::alsS::ilvC::ilvD, inserted at BamHI and NotI restriction sites; Kanr | This study |
| pCT02 | pCOLA pT7::kivd::adh2, inserted at NdeI and XhoI restriction sites; Kanr | This study |
| pCT03 | pCOLA pT7::alsS::ilvC::ilvD::pT7::kivd::adh2; Kanr | This study |
| pCT06 | pCOLA pT7::alsS::ilvC::ilvD::pT7::adhE; Kanr | This study |
| pCT13 | pCOLA pT7::alsS::ilvC::ilvD::pT7::kivd::adhE; Kanr | This study |
| pET24b adhE | pET24b pT7::adhE; Kanr | This study |
| pPC20 | FLP+, λ cI857+, λ pR Rep(Ts); Ampr Cmr | 11 |