E. coli strains |
|
|
BW25113 |
K-12; λ−; F−lacIqrrnBT14 ΔlacZWJ16hsdR514 ΔaraBADAH33 ΔrhaBADLD78
|
12 |
JW0855 |
BW25113, poxB::Kanr
|
6 |
JW2294 |
BW25113, pta::Kanr
|
6 |
JW1841 |
BW25113, zwf::Kanr
|
6 |
JW3205 |
BW25113, mdh::Kanr
|
6 |
JW1375 |
BW25113, ldhA::Kanr
|
6 |
JW4115 |
BW25113, frdA::Kanr
|
6 |
JW1095 |
BW25113, ndh::Kanr
|
6 |
JW1228 |
BW25113, adhE::Kanr
|
6 |
BFA7.001(λDE3) |
BW25113, λDE3 Δzwf Δmdh ΔfrdA Δndh Δpta ΔpoxB ΔldhA::Kan |
This study |
BFA8.001(λDE3) |
BW25113, λDE3 Δzwf Δmdh ΔfrdA Δndh Δpta ΔpoxB ΔldhA ΔadhE::Kan |
This study |
BL21(DE3) |
F−ompT hsdSB(rB− mB−) gal dcm (λDE3) |
Invitrogen (catalog no. C600003) |
Plasmids |
|
|
pSA55 |
ColE1 ori, plac::kivd::adh2; Ampr
|
3 |
pSA69 |
ColE1 ori, plac::alsS::ilvC::ilvD; Kanr
|
3 |
pCOLADuet |
ColA ori; Kanr
|
Novagen (catalog no. 71406-3) |
pET24b |
f1 ori; Kanr
|
Novagen (catalog no. 69750-3) |
pCT01 |
pCOLA pT7::alsS::ilvC::ilvD, inserted at BamHI and NotI restriction sites; Kanr
|
This study |
pCT02 |
pCOLA pT7::kivd::adh2, inserted at NdeI and XhoI restriction sites; Kanr
|
This study |
pCT03 |
pCOLA pT7::alsS::ilvC::ilvD::pT7::kivd::adh2; Kanr
|
This study |
pCT06 |
pCOLA pT7::alsS::ilvC::ilvD::pT7::adhE; Kanr
|
This study |
pCT13 |
pCOLA pT7::alsS::ilvC::ilvD::pT7::kivd::adhE; Kanr
|
This study |
pET24b adhE
|
pET24b pT7::adhE; Kanr
|
This study |
pPC20 |
FLP+, λ cI857+, λ pR Rep(Ts); Ampr Cmr
|
11 |