Table 2.

Effects of deleting anaerobic pathways on feasibility of producing isobutanol as the sole product

Deletionsa	No. of EMs based on:				Range of iBuOH yield on glucose (g/g)
	Total	Biomass	iBuOHb	Biomass and iBuOH	Minimum	Maximum
Δfrd ΔldhA Δpta ΔpoxB ΔadhE	14	0	14	0	0.41	0.41
Δfrd ΔldhA Δpta ΔpoxB	1,150	900	814	282	0.04	0.41
Δfrd ΔldhA ΔadhE	14	0	14	0	0.41	0.41
Δfrd Δpta ΔpoxB ΔadhE	28	0	14	0	0.41	0.41
ΔldhA Δpta ΔpoxB ΔadhE	427	251	427	251	0.06	0.41
Δfrd ΔadhE	28	0	14	0	0.41	0.41

^aΔfrd, removal of succinate-producing pathway; ΔldhA, removal of lactate-producing pathway; Δpta ΔpoxB, removal of acetate-producing pathway; ΔadhE, removal of ethanol-producing pathway.

^biBuOH, isobutanol.