Table 3.

Elementary mode analysis for the effects of multiple deleted genes applied to different designed E. coli strains, including JCL260 pSA55/pSA69 and BFA7.001(DE3) pCT03 or BFA7.001(DE3) pCT13^a under anaerobic conditions

Deleted gene or summary value	Result for strain
	JCL260 pSA55/pSA69	BFA7.001(DE3) pCT03
Deleted genes
ΔadhE	+
Δfnr	+
ΔpflB	+
ΔfrdBC	+	+
Δpta	+	+
ΔldhA	+	+
Δndh		+
ΔpoxB		+
Δzwf		+
Δmdh		+
ΔaceF		+
Total no. of anaerobic EMs	14	6
No. of biomass-producing EMs	0	2
No. of isobutanol-producing EMs	14	6
Isobutanol yield (g/g)	0.41	0.29–0.41

^aInformation on JCL260 pSA55/pSA69 is from reference 3, and that for BFA7.001(DE3) pCT03 and BFA7.001(DE3) pCT13 is from this study.