Table 4.
Kinetic parameters for the endogeneous alcohol/aldehyde dehydrogenase AdhE reacting with acetaldehyde, isobutyraldehyde, or butyraldehydea
| Substrate | Km (μM) | kcat (s−1) | kcat/Km (103) (mM−1 s−1) |
|---|---|---|---|
| Acetaldehyde | 48.6 ± 3.5 | 5.72 ± 0.07 | 118 ± 7 |
| Isobutyraldehyde | 54.3 ± 5.1 | 2.13 ± 0.05 | 39.5 ± 2.8 |
| Butyraldehyde | 196 ± 38 | 59.2 ± 8.1 | 305 ± 17.1 |
a
Each value is a mean ± average deviation from two independent measurements. Nonlinear regression (SigmaPlot) was used to determine Km and kcat values with P values of <0.05. The R2 coefficients for nonlinear regression were greater than 0.99 for all cases.