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Schematic of “high-resolution” metagenomics. (a) Environmental sample, top layer of the sediment (boxed) known for high rates of methane metabolism; (b) microcosm incubations with labeled substrates; (c) separation of heavy DNA via isopycnic centrifugation; (d) DNA purification; (e) sequencing and assembly; (f) bioinformatic analysis; (g) phylogenetic assignment/binning; (h) analysis of individual (composite) genomes.
