Table 2.
Primers used for amplifications and sequencing
| Primer | Sequence (5′-3′) | Gene product | Size of PCR amplicon (bp) | Size of the target sequence (bp) | Annealing temp (°C) | Reference |
|---|---|---|---|---|---|---|
| gyrB_F | GGGGTCTACTGCTTCACCAA | DNA gyrase, β subunit | 669 | 477 | 59 | This study |
| gyrB_R | CTTGTCCGGGTTGTACTCGT | |||||
| groL_F | CAAGGAAGTTGCTTCCAAGG | Chaperonin GroEL | 782 | 510 | 56 | This study |
| groL_R | CATCGATGATGGTGGTGTTC | |||||
| gltA_F | TTCCGTCTGCTCTCCAAGAT | Citrate synthase I | 626 | 495 | 58 | This study |
| gltA_R | TTCATGATGATGCCGGAGTA | |||||
| metG_F | TGGCAACTGATCCTCGTACA | Methionyl-tRNA synthetase | 657 | 504 | 57 | This study |
| metG_R | TCTTGTTGGCCATCTCTTCC | |||||
| ppsA_F | AGTCCAACGAGTACGCCAAC | Phosphoenolpyruvate synthase | 619 | 537 | 60 | This study |
| ppsA_R | TCGGCCAGATAGAGCCAGGT | |||||
| recA_F | AGAACAAACAGAAGGCACTGG | Recombinase A | 640 | 561 | 57 | This study |
| recA_R | AACTTGAGCGCGTTACCAC | |||||
| ahh1_F | GCCGAGCGCCCAGAAGGTGAGTT | Extracellular hemolysin | 130 | 60 | 74 | |
| ahh1_R | GAGCGGCTGGATGCGGTTGT | |||||
| asa1_F | TAAAGGGAAATAATGACGGCG | Hemolysin | 249 | 56 | 74 | |
| asa1_R | GGCTGTAGGTATCGGTTTTCG | |||||
| act_F | AGAAGGTGACCACCAAGAACA | Cytotoxic enterotoxin | 232 | 56 | 33 | |
| act_R | AACTGACATCGGCCTTGAACTC | |||||
| ast_F | TCTCCATGCTTCCCTTCCACT | Heat-stable cytotonic enterotoxin | 331 | 60 | 58 | |
| ast_R | GTGTAGGGATTGAAGAAGCCG | |||||
| ascV_F | CTCGAACTGGAAGAGCAGAATG | Type III secretion system inner | 577 | 60 | This study | |
| ascV_R | GAACATCTGGCTCTCCTTCTCGATG | membrane component | ||||
| eno_F | CGCCGACAACAACGTCGACATC | Enolase | 598 | 60 | This study | |
| eno_R | CTTGATGGCAGCCAGAGTTTCG | |||||
| aexT_F | ATGCAGATTCAAGCAAACAC | ADP-ribosylating toxin | 226 | 54 | This study | |
| aexT_R | TTGCCGATCCACTCTTTGAT |