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Cross-feeding agar diffusion assay of the E. coli BLic5ΔA1ΔFGEHI and BLic5ΔA2ΔFGEHI mutants with the Bliα (BLic5ΔA2) and Bliβ (BLic5ΔA1) peptides. The bacterial activity restored between BLic5ΔA1 and BLic5ΔA2ΔFGEHI suggested that this mutant was able to produce the Bli〈 peptide. Similarly, the M. luteus inhibition zone observed between BLic5ΔA2 and BLic5ΔA1ΔFGEHI suggested that this mutant was able to produce the Bliβ peptide.
