Table 1.

List of E. coli strains used in the present study

Strain	Fosmid	Observation(s)a
BLic5	pLic5	E. coli BL21-Gold (DE3) cells transformed with the pLic5 fosmid containing the complete lichenicidin gene cluster; production of Bliα and Bliβ
BLic5ΔA1	pLic5ΔA1	Deletion of licA1 gene in the pLic5 fosmid; single production of Bliβ
BLic5ΔA2	pLic5ΔA2	Deletion of licA2 gene in the pLic5 fosmid; single production of Bliα
BLic5ΔFGEHI	pLic5ΔFGEHI	Replacement of licFGEHI genes in the pLic5 fosmid by an Aprar cassette; production of Bliα and Bliβ
BLic5ΔA1ΔFGEHI	pLic5ΔA1ΔFGEHI	Replacement of licFGEHI genes in the pLic5ΔA1 fosmid by an Aprar cassette; single production of Bliβ
BLic5ΔA2ΔFGEHI	pLic5ΔA2ΔFGEHI	Replacement of licFGEHI genes in the pLic5ΔA2 fosmid by an Aprar cassette; single production of Bliα
BWLic5ΔFGEHI	pLic5ΔFGEHI	E. coli BW25113 cells transformed with the pLic5ΔFGEHI fosmid
BWΔtolCLic5ΔFGEHI	pLic5ΔFGEHI	E. coli BW25113ΔtolC::kan cells transformed with the pLic5ΔFGEHI fosmid

^aApra^r, apramycin resistance.