Phylogenetic analysis (rooted neighbor-joining tree) of SidL homologs (A) and sequence alignment of the AlcB domains of selected SidL homologs (B). (A) Species abbreviations are as follows: Af, Aspergillus fumigatus; Afl, Aspergillus flavus; An, Aspergillus nidulans; Ao, Aspergillus oryzae; At, Aspergillus terreus; Av, Azotobacter vinelandii; Ec, Escherichia coli; Gz, Gibberella zeae; Mg, Magnaporthe grisea; Mt, Mycobacterium tuberculosis; Nc, Neurospora crassa; Pb, Paracoccidioides brasiliensis; Pc, Penicillium chrysogenum; Pm, Penicillium marneffei; Psa, Pseudomonas aeruginosa; Psp, Pseudomonas putida; Re, Ralstonia eutropha; Sp, Schizosaccharomyces pombe; Um, Ustilago maydis. (B) The sequence numbering refers to SidL. Invariant residues (white letters on a black background) and residues conserved in three of the four sequences (shaded) are indicated. A. fumigatus SidL displays 43% sequence identity to E. coli IucB. The PSI-BLAST homology search with SidL revealed E values of 2e−35, 2e−39, and 3e−11 for IucB, SidF, and RV1347c, respectively.