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sidL expression is regulated neither by SreA nor by iron availability. For Northern blot analysis, A. fumigatus wt and ΔsreA strains were grown for 24 h in liquid flask cultures under iron-depleted (−Fe) and iron-replete (+Fe) conditions. The mirB gene, which encodes a putative siderophore transporter (29), was used as a control for the regulation of iron and SreA. Ethidium bromide-stained rRNA is shown as control for loading and RNA quality.
