Fig. 2.

Production of MPBD in wild-type and stlA-null strains. Shown is the time course of secreted MPBD. The wild-type or stlA⁻ null strain was developed for the indicated times. The structures were harvested and dissociated in 20 mM phosphate buffer, pH 6.2. The MPBD present in the supernatant was concentrated using the hydophobic resin Amberlite XAD-2, eluted with methanol, and then quantified using a mass spectrometer coupled to a C₁₈ high-performance liquid chromatography (HPLC) column. The averages of 3 experiments are shown.