Table 2.
Induction of SDF-1 production under submerged conditionsa
| Addition to KP cells | SDF-1 release |
|---|---|
| None | No |
| MPBD | Yes |
| MPBD + mPKI | Yes |
| MPBD + TPCK | No |
| MPBD + anti-protease | No |
| SDF-1 | Yes |
| SDF-1 + mPKI | No |
| SDF-1 + anti-protease | No |
| SDF-1 + TPCK | No |
| LiCl | Yes |
| LiCl + mPKI | Yes |
| Gsk3B inhibitor VIII | Yes |
| Gsk3B inhibitor VIII + mPKI | Yes |
| mGSKI | Yes |
| mGSKI + mPKI | Yes |
a
KP cells were deposited in 24-well plates at a density of 103/cm2 and incubated overnight in starvation buffer containing 5 mM cAMP. SDF-1 production was induced with 10 nM MPBD, 10 pM synthetic SDF-1, 5 mM LiCl, 100 nM Gsk3B inhibitor VIII, or 40 μM myristylated Gsk3B peptide inhibitor. Myristilated PKA inhibitor (mPKI) (20 μM) was added 45 min before induction when indicated. Anti-protease antibodies (final dilution, 1/5,000) or TPCK (20 μM) were added just prior to induction, as indicated. No indicates that less than 10 units per 103 cells were detected. Yes indicates that 103 units of SDF-1 per 103 cells or more were detected. Each assay was repeated at least three times.