Table 4.
SDF-1 production in mixed populations of developed cellsa
| Strain | crlA− | stlA− | gpaA− | acgA− |
|---|---|---|---|---|
| crlA− | − | + | − | + |
| stlA− | − | + | + | |
| gpaA− | − | + | ||
| acgA− | − |
a
Vegetative cells of the indicated strains were harvested, washed, and developed either alone or as a 50/50 mixture with the one of the other strains as shown. The fruiting bodies were collected in starvation buffer at the end of development (24 to 26 h). After the cells were spun down, an aliquot of the supernatant was harvested to measure the amount of SDF-1 produced. A minus indicates that less than 10 units per 103 cells were detected; a plus indicates that 500 units of SDF-1 per 103 cells or more were detected. Each assay was repeated at least three times.