Fig. 2.

Dot blot analysis of β-(1,6) glucan in alkali-extracted whole-cell lysate from 10 OD₆₀₀ units of wild-type and ccr4Δ mutant cultures grown for 24 h in YPD broth at the indicated temperatures. The dots represent 5 μl of alkali extraction from equivalent lysates normalized by protein content. β-(1,6) Glucan was detected using a rabbit anti-β-(1,6) glucan polyclonal antiserum, followed by an anti-rabbit IRDye-700-conjugated secondary antibody and by imaging on a Li-Cor Odyssey scanner. The bar graph represents the quantified intensities of the dots ± standard errors of the mean (SEM). The blot is representative of at least 3 independent experiments.