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(A) Northern blot time course of RNA from the wild type and the ccr4Δ mutant harvested during a shift from 30°C to 37°C and probed with KAR2 or OST2. rRNA was provided as a reference for loading. (B) qRT-PCR analysis of SSS1, SEC16, ALG7, KAR2, and OST2 expression in response to 37°C (shifted for 1 h), DTT (10 mM for 30 min), or tunicamycin (10 μg/ml for 30 min.). Each value is normalized to matched, untreated controls. The error bars represent SEM for triplicate reactions.
