Fig. 1.

The absence of Kar9 and Bim1 does not significantly affect maximal hyphal growth and nuclear movements. (A) Radial growth of wild-type, kar9Δ, and bim1Δ strains after 5 days at 30°C on full medium (AFM). (B) Nuclear dynamics in wild-type, kar9Δ, and bim1Δ hyphae with H4-GFP-labeled nuclei. Images of selected time-lapse movies are presented (green, H4-GFP; red, DIC). Yellow circles show examples of backward nuclear movements, whereas white circles represent forward events. Time is indicated in minutes. Bars, 5 μm. (C) Dynamic distributions of nuclei. Distances between neighboring nuclei were measured in the wild type and the promoter mutants and plotted in four different size ranges. (D) Distances between the hyphal tip and the first nucleus (N) were measured in the wild type and the kar9Δ strain.