Fig. 2.

Dynein is the motor for forward and backward nuclear movements. (A) Radial growth of the wild type and strains with shortened DYN1 promoters on full medium (AFM) for 4 days at 30°C. prom180 and prom130 indicate the lengths (in base pairs) of the remaining DNA sequence upstream of the start codon. (B) Nuclear distribution in the dyn1Δ, prom180-DYN1, and prom130-DYN1 mutants. Images of time-lapse movies are shown (green, H4-GFP; red, DIC). Time is indicated in minutes. Note the dense nuclear cluster in the tip region of the dyn1Δ hyphae and the loose nuclear aggregate in the tip region of the prom130-DYN1 hyphae. Bars, 5 μm. (C) Nuclear clustering index of the dyn1Δ and the DYN1 promoter mutants. The average number (nb) of nuclei within 15 hyphal segments, each 15 μm long, showing a higher nuclear content was determined in the different mutants and compared with the average density of nuclei in randomly picked 15-μm-long regions in wild-type hyphae. Analyses were performed with images as described for panel B. Bars indicate standard deviations.