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. 2011 Jul;10(7):985–997. doi: 10.1128/EC.05025-11

Fig. 7.

Fig. 7.

T. brucei TbGNA1 conditional null mutant: confirmation of gene essentiality in culture. (A) Growth curve for TbGNA1 conditional null mutant cells, subcultured every day, grown under permissive (plus tetracycline) conditions. (B) Growth curve for TbGNA1 conditional null mutant cells, subcultured every day, grown under nonpermissive (minus tetracycline) conditions. (C) Ethidium bromide-stained agarose gel of reverse transcription-PCR products from RNA extracted from TbGNA1 conditional null mutant cells after 0, 6, 24, and 48 h and 10 days without tetracycline, as indicated. The middle panel shows reverse transcription-PCR products using TbGNA1 primers, the upper panel is a control without reverse transcriptase, and the lower panel is a control using DPMS primers to show equal RNA input.