Table 2.
S. thermophila GH ORFs for which a specific enzyme activity could be indicated in the functional screen
| Parameter | Result on substrate: |
|||
|---|---|---|---|---|
| CMC | β-Glucan | Xylan | Starch | |
| No. of active fosmid clones | 24 | 21 | 21 | 9 |
| Mapped ORFs from activity screen | ADN02558 | ADN02334a | ADN02449 | ADN02538 |
| ADN02402b | ADN02324a | ADN01802 | ADN02534 | |
| ADN02400b | ADN01802a | ADN02828 | ||
| ADN02392b | ||||
| ADN02996 | ||||
| ADN02997 | ||||
| ADN02998 | ||||
| ADN02999 | ||||
| Total no. of ORFs mapped | 8 | 2 | 3 | 2 |
| No. of in silico predicted ORFs to have activity shown | >15 | >15 | 8 | 7 |
a
The fosmid clones carrying this ORF showed β-glucanase but not CMCase activity in E. coli.
b
The fosmid clones carrying this ORF showed CMCase but not β-glucanase activity in E. coli.