Table 3.
S. thermophila GH ORFs which carry the new CBM (StX), their modular organization, and experimentally determined activities of the recombinantly expressed proteinsa
| ORF | Modular structure | Known activities for GH domain by sequence similarity | Substrate(s) of recombinant enzyme(s) |
|---|---|---|---|
| ADN02703 | SP-GH10-TPS-StX | Xylanase, β-1,3 endoxylanase | Xylan (oat spelt) |
| ADN02189 | SP-GH5-TPS-StX | Cellulase, β-1,4 endoglucanase, others | ND |
| ADN01378 | SP-GH12-TPS-StX | Endoglucanase, xyloglucan hydrolase, others | ND |
| ADN02996 | SP-GH5-TPS-StX | Cellulase, β-1,4 endoglucanase, others | CMC, not β-glucan |
| ADN02997 | SP-GH9-CBM3-TPS-StX | Endoglucanase, cellobiohydrolase | β-Glucan, not CMC |
| ADN02998 | SP-GH9-CBM3-TPS-StX | Endoglucanase, cellobiohydrolase | ND |
| ADN02999 | SP-GH12-TPS-StX | Endoglucanase, xyloglucan hydrolase, others | CMC and β-glucan |
a
Abbreviations: SP, signal peptide; TPS, TPS-rich linker peptide; StX, module StX; CMC, carboxymethylcellulose; ND, not determined.