Table 2.
Effect of 2% NaCl on the chitinolytic activity of marine-derived strains O.Y. 3807 and O.Y. 1607 and the terrestrial strain T203a
Isolate and activity type | 4-MU-(GlcNAc) |
4-MU-(GlcNAc)2 |
||||
---|---|---|---|---|---|---|
Chitinolytic act (nmol MU/min/μg proteins) |
Inhibition (%) | Chitinolytic act (nmol MU/min/μg proteins) with |
Inhibition (%) | |||
Control | NaCl (2%) | Control | NaCl (2%) | |||
Intracellular | ||||||
T203 | 1.52 ± 0.01 | 0.86 ± 0.05 | 43 | 0.13 ± 0.04 | 0.03 ± 0.008 | 79 |
O.Y. 3807 | 1.06 ± 0.14 | 0.22 ± 0.05 | 79 | 0.03 ± 0.01 | 0.003 ± 0.05 | 89 |
O.Y. 1607 | 1.36 ± 0.18 | 0.85 ± 0.17 | 38 | 0.13 ± 0.004 | 0.03 ± 0.01 | 77 |
Extracellular | ||||||
T203 | 0.1 ± 0.007 | 0.01 ± 0.004 | 84 | 0.013 ± 0.004 | 0.003 ± 0.001 | 77 |
O.Y. 3807 | 0.04 ± 0.002 | 0.004 ± 0.00 | 91 | 0.008 ± 0.001 | 0.002 ± 0.007 | 70 |
O.Y. 1607 | 0.13 ± 0.02 | 0.027 ± 0.004 | 80 | 0.011 ± 0.007 | 0.002 ± 0.007 | 77 |
Results (± standard deviations) are averages from three independent experiments with three replicates for each isolate and treatment.