Fig. 4.

Hydrolysis of increasing amounts of xylan substrates by R. albus 8 endoxylanases in the presence of two accessory enzymes (β-xylosidase and α-l-arabinofuranosidase). (A) Thin-layer chromatography of products of hydrolysis by R. albus 8 xylan-hydrolyzing enzymes. In each lane other than the control, where enzymes were not added, the enzymes were added as a mixture of endoxylanases (Xyn10A, Xyn10B, Xyn11D, Xyn11E, and Xyn11F at 0.1 μM each), β-xylosidase (Xyl3A at 0.5 μM), and α-l-arabinofuranosidase (Ara51A at 0.5 μM) to the indicated substrates at concentrations ranging from 0.5% to 8%.The hydrolysis products (0.5 μl) were resolved by TLC with xylo-oligosaccharides (X1 to X5) and arabinose (A1) as standards. (B) Quantification of products in the experiment shown in panel A with a reducing sugar assay. The reducing sugar concentrations were calculated from the absorbance at 410 nm with comparison to a standard curve generated with known concentrations of glucose.