Fig. 5.

Optimization of xylan (soluble WAX) hydrolysis with the five endoxylanases and their accessory enzymes from R. albus 8. (A) In lane 1 is substrate without enzymes to serve as a control experiment. The experiment in Fig. 4 with 8% of the appropriate substrate was repeated in lane 2, the concentrations of the multiple xylanases were increased (2.5 μM) in lane 3, the β-xylosidase concentration was increased (2.5 μM) in lane 4, the α-l-arabinofuranosidase concentration was increased (2.5 μM) in lane 5, α-glucuronidase was added (0.5 μM) in lane 6, and both the multiple endoxylanases and β-xylosidase were increased in lane 7. The products of hydrolysis were resolved by TLC with arabinose (A1) and xylo-oligosaccharides (X1 to X5) as standards. (B) Quantification of monosaccharides released during optimization of soluble wheat arabinoxylan hydrolysis by R. albus 8 enzymes. The products of hydrolysis presented in the TLC analysis were identified by comparison of peaks with retention times of standards arabinose (A1) and xylo-oligosaccharides (X1 to X3). Standard curves were derived to estimate the amounts of monosaccharides released in each experiment. All experiments were performed three times, and representative curves were shown. The values of monosaccharides released in panel B represent the means of three experiments ± standard errors.