Table 1.
Tb3+ concn (μM) | % spore germination in 2.5 h |
|
---|---|---|
Intact spores | Decoated spores | |
0 | 100 | 100 |
50 | 100 | 56 |
500 | 92 | 32 |
2,500 | 83 | 21 |
Intact and chemically decoated spores of C. perfringens were germinated with dodecylamine plus various TbCl3 concentrations, and the extent of germination was assessed by monitoring DPA release fluorometrically as described in the text. Use of 2.5 mM TbCl3 in the fluorometric DPA assay did not alter the fluorescence compared to that with the standard 50 μM TbCl3 normally present.