Table 3.
Effects of various multivalent cations on the valine germination of intact and decoated wild-type B. subtilis sporesa
| Cation | % spore germination in: |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Intact spores at cation concn of: |
Decoated spores at cation concn of: |
|||||||||
| 0 | 50 μM | 500 μM | 2.5 mM | 5 mM | 0 | 50 μM | 500 μM | 2.5 mM | 5 mM | |
| Gd3+ | 96 | 92 | 94 | 84 | 85 | 0 | 0 | 0 | ||
| Dy3+ | 96 | 92 | 94 | 96 | 84 | 0 | 0 | 0 | ||
| Ca2+ | 94 | 88 | 92 | 88 | 82 | 72 | 64 | 60 | ||
| Mg2+ | 94 | 88 | 90 | 82 | 82 | 76 | 64 | 48 | ||
| Zn2+ | 94 | 90 | 88 | 20 | 82 | 0 | 0 | 0 | ||
Intact and chemically decoated wild-type B. subtilis spores (PS533) were germinated for 2.5 h at 37°C with 2 mM l-valine plus various concentrations of divalent and trivalent cation chlorides, and the percentages of germinated spores were determined by phase-contrast microscopy as described in the text.