Table 1.
Strains and plasmids used
| Strain(s) or plasmid | Characteristics | Reference or source |
|---|---|---|
| Strains (abbreviations) | ||
| DH5α | E. coli cloning strain | Gibco-BRL |
| F113rif (F113) | P. fluorescens wild type; Rifr | 36 |
| F113gacS | F113rif gacS mutant Rifr Kmr | 26 |
| F113sadB | F113rif sadB mutant Rifr Kmr | 29 |
| F113kinB | F113rif kinB mutant Rifr Kmr | This work |
| F113wspR | F113rif wspR mutant Rifr Spcr | 29 |
| F113algB | F113rif algB mutant Rifr Kmr | This work |
| F113gacS-kinB (KG) | F113rif kinB mutant gacS Rifr Kmr Gnr | This work |
| F113kinB-sadB (KS) | F113rif kinB mutant sadB Rifr Gnr Kmr | This work |
| F113gacS-sadB-wspR (GSW) | F113rif sadB mutant wspR gacS Rifr Kmr Spcr Gnr | 29 |
| F113kinB-sadB-wspR (KSW) | F113rif sadB mutant wspR kinB Rifr Kmr Spcr Gnr | This work |
| F113kinB(pBG1708) | F113rif kinB mutant pLFAR3kinB Rifr Kmr Tcr | This work |
| F113v35 (V35) | Hypermotile phenotypic variant | 25 |
| F. oxysporum f. sp. radicis-lycopersici | Tomato isolate | IPO-DLO, Wageningen, Netherlands |
| P. cactorum CH971, CH972, CH1020, CH1021 | Strawberry pathogen | IFAPA, Málaga, Spain |
| Plasmids | ||
| pGEM-T Easy vector | Cloning vector; Ampr | Promega |
| pK18mobsacB | Suicide vector; sacB Kmr | 34 |
| pG18mob2 | Suicide vector; Gnr | 23 |
| pBG1708 | pLAFR3 derivative containing the algB and kinB genes; Tcr | This work |
| pRK2013 | Helper plasmid; Kmr | 19 |
| pRK600 | Helper plasmid; Cmr | 20 |