Skip to main content
. 2011 Aug;77(15):5505–5512. doi: 10.1128/AEM.02070-10

Fig. 2.

Fig. 2.

Construction of the double deletant SB-62 (ΔtrpB ΔpurA). (A to C) Southern hybridization analyses of PstI-digested genomic DNA of strains derived from R. marinus PRI 493 (lanes 1 to 5). Lane M, HindIII-digested λ DNA as a size marker. The positions (in kilobases) of molecular size markers (from 2.0 to 9.4 kb) are shown to the left of the gel shown in panel A. The membrane was probed three times with digoxigenin-labeled fragments corresponding to trpB (A), purA (B), and the ori fragment from pUC19 (C). For detection of the size marker, labeled λ DNA was added with each probe. (D) The strategy used for generating the double deletant SB-62 (ΔtrpB ΔpurA), using both the trpB and purA selective markers. The phenotypes and genotypes are indicated for each strain. Nonsel., nonselective.