Table 1.

Bacterial strains and plasmids used in this study

Bacterial strain or plasmid	Relevant phenotype or genotypea	Reference or source
E. coli strains
S17-1 λpir	Tmpr SmrrecAthi pro hsd(r− m+) RP4:2-Tc:Mu:Km::Tn7 λpir	33
K-12 MG1655	Wild type; ATCC 700926	ATCC
K-12 Rifr	MG1655 Rifr	27
K-12 Nalr	MG1655 Nalr	27
K-12 Nal::gfp	K-12 Nalr containing mini-Tn5-PA1–04/03::gfpmut3 cassette; Nalr Kmr	27
MG1655 csrA	csrA::mini-Tn5 Kmr	35
Plasmids
pB10	IncP-1β Tcr Smr Amxr Sulr Hgr	61
pB10::rfp	pB10 with mini-Tn5-Km-PA1-04/03::rfp	27
pB10::T7cat	pB10 with mini-MaT7cat	This work
pMaT7cat	Mini-mariner transposon containing T7 RNA polymerase; Cmr Gmr	42
pB2a-Evoglow	pGem3Zf+ with pT7-Evoglow lacp-dsRedcat cassette in PvuII site; reporter plasmid; Ampr Cmr	This work
pG-RFP	pGem3Zf+ with pT7-dsRedcat in SmaI site; reporter plasmid; Ampr Cmr	This work
pG-GFP	pGem3Zf+ with pT7-gfpmut3 in SmaI site; reporter plasmid; Ampr	This work

^aTmp^r, Sm^r, Amp^r, Cm^r, Gm^r, Tc^r, Amx^r, Sul^r, Km^r, Rif^r, Nal^r, and Hg^r, trimethoprim, streptomycin, ampicillin, chloramphenicol, gentamicin, tetracycline, amoxicillin, sulfonamide, kanamycin, rifampin, nalidixic acid, and mercury ion resistance phenotypes, respectively.