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. 2011 Aug;77(15):5132–5140. doi: 10.1128/AEM.05008-11

Fig. 2.

Fig. 2.

Effect of fucO overexpression in LY180. (A) In vitro NADPH-dependent reduction of furfural and 5-HMF by cell extracts of LY180 containing vector alone (pTrc99A) and IPTG-induced fucO in LY180(pLOI4319). (B) In vitro NADH-dependent reduction of furfural and 5-HMF by cell extracts of LY180(pTrc99A) and IPTG-induced fucO in LY180(pLOI4319). In panels A and B, open bars represent activity with furfural (10 mM) as a substrate. Gray bars represent activity with 5-HMF (10 mM). (C) In vivo furfural (10 mM) reduction by chloramphenicol-inhibited, nongrowing cells (LY180, 0.88 mg cell dry weight ml−1) containing vector alone (pTrc99A) or 0.1 mM IPTG-induced fucO in LY180(pLOI4319). (D) Growth inhibition of LY180(pTrc99A) with vector alone (▵, 0 mM IPTG) or pLOI4319 (○, 0 mM IPTG; •, pLOI4319 induced with 0.1 mM IPTG).