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Effects of medium supplements and fucO expression on furfural tolerance. Strains LY180(pTrc99A) and LY180(pLOI4319) were grown for 48 h in tube cultures containing AM1 medium, 15 mM furfural (A and B), or 10 mM furfural (C). IPTG (0.1 mM) was also included with LY180(pLOI4319) to induce fucO. Bars indicate the presence (solid) or absence (open) of supplement. (A) Yeast extract (1 g liter−1). (B) Cysteine (0.1 mM). (C) AM1-glucose (50 g liter−1) replacing xylose.
