Fig. 5.

Effect of fucO expression on furfural tolerance during d-lactate production from xylose. Batch fermentations were conducted in pH-controlled fermentation vessels in the absence and presence of furfural. Expression of fucO from pLOI4319 (0.1 mM IPTG) was compared to that for vector controls (pTrc99A) using XW068 as the host. Controls were included without furfural (open symbols and dotted lines). (A) Cell mass (10 mM furfural). (B) d-Lactate (10 mM furfural). (C) Furfural (10 mM furfural). (D) Cell mass (15 mM furfural). (E) d-Lactate (15 mM furfural). (F) Furfural (15 mM furfural).