Table 2.

Sequence variation at six loci

Locus	Targeta	Fragment (gene)b size (bp)	No. of alleles	Avg GC content (%)	Ksc	Kad	Ka/Ks	πe	No. (%) of polymorphic sites
									Total	Toxic	Nontoxic
adk	Bsph_4593	531 (654)	8	39.5	0.1013	0.0075	0.0740	0.0249	77 (14.5)	2 (0.4)	72 (13.6)
ccpA	Bsph_4200	579 (1,005)	8	40.4	0.3192	0.0059	0.0185	0.0428	132 (22.8)	1 (0.2)	128 (21.6)
pycA	Bsph_1392	534 (3,435)	9	42.0	0.3608	0.0086	0.0238	0.0480	133 (24.9)	2 (0.4)	128 (24.0)
glyA	Bsph_1005	552 (1,242)	9	40.2	0.2419	0.0054	0.0223	0.0414	117 (21.2)	3 (0.6)	105 (19.0)
glcK	Bsph_1259	654 (909)	12	37.4	0.3872	0.0463	0.1196	0.0715	250 (38.2)	6 (0.9)	235 (36.0)
glpF	Bsph_0241	414 (804)	11	39.0	0.2488	0.0164	0.0659	0.0496	107 (25.8)	7 (1.7)f	104 (25.1)

^aBased on B. sphaericus C3-41 genome (GenBank accession number NC_010382; Hu et al. [6]).

^bValues in parentheses represent the total length of the gene.

^cK_s, number of synonymous changes per synonymous site.

^dK_a, number of nonsynonymous changes per nonsynonymous site.

^eπ, nucleotide diversity per site.

^fToxic strain LP1-G shared an identical glpF gene fragment with nontoxic strain Dak614 and, therefore, was not included in the analysis.