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. 2011 Aug;77(15):5089–5099. doi: 10.1128/AEM.00112-11

Fig. 2.

Fig. 2.

P. aeruginosa PAO1 motility and biofilm assays. (A) Altered swarming motility of P. aeruginosa PAO1 on swarming plates supplemented with crude cell extracts of cosmids pWEB-TNC-B2, -C6, -A5, -B9, and -G2. Plates were supplemented with 5 to 100 μg of crude cell extracts of E. coli harboring the QQ cosmids. The plates were inoculated with 1 × 108 cells of P. aeruginosa PAO1 and incubated overnight. (B) Biofilm assays using sterile test tubes filled with LB medium and supplemented with 5 to 100 μg of crude cell extracts of cosmid clones. The tubes were inoculated with 1 × 108 cells of P. aeruginosa PAO1 and incubated at 37°C and 140 rpm for 6 to 8 h. Controls in panels A and B were either E. coli cells or E. coli harboring a negatively tested pWEB-TNC cosmid clone inoculated with PAO1. For overview purposes, only one control is displayed.