Table 1.
Bacterial strains, plasmids, and constructs used in this study
| Strain or construct | Description | Reference(s) or source |
|---|---|---|
| EPI100-T1 phage T1-resistant E. coli | F−mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74recA1 endA1 araD139 Δ(ara leu)7697galU galK λ−rpsL nupG | Epicentre Technologies, Madison, WI |
| E. coli XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1lac [F′ proABlacIqZΔM15 Tn10 (Tetr)] | Stratagene, La Jolla, CA |
| E. coli BL21(DE3) | F−ompT hsdSB (rB− mB−) gal dcm(DE3) | Novagen, Darmstadt, Germany |
| Rhizobium sp. strain NGR234 | Wild-type New Guinea isolate; Rifr | 47 |
| Pseudomonas aeruginosa PAO1 | Wild-type strain of P. aeruginosa; Ampr | 19 |
| Agrobacterium tumefaciens NTL4(pCF218)(pCF372) | Reporter strain for AHL detection; traI::lacZ Tetr Spr | 14, 15, 25 |
| Chromobacterium violaceum CV026 | Reporter strain for autoinducer I; mini-Tn5 in cviI | 27 |
| pWEB-TNC | Cosmid cloning vector derived from pWE15; linearized with SmaI; ColE1; cos site; T7 promoter; Ampr Cmr | Epicentre Technologies, Madison, WI |
| pWEB-TNC-A5 | pWEB-TNC with a 40.4-kb insert from NGR234 | This study |
| pWEB-TNC-B2 | pWEB-TNC with a 34.0-kb insert from NGR234 | This study |
| pWEB-TNC-B9 | pWEB-TNC with a 42.0-kb insert from NGR234 | This study |
| pWEB-TNC-C6 | pWEB-TNC with a 37.7-kb insert from NGR234 | This study |
| pWEB-TNC-G2 | pWEB-TNC with a 33.3-kb insert from NGR234 | This study |
| pET21a | E. coli His6-tagged expression vector; Ampr | Novagen, Darmstadt, Germany |
| pET21a::dlhR | pET21a containing dlhR gene cloned into NdeI and HindIII restriction sites | This study |
| pET21a::qsdR1 | pET21a containing qsdR1 gene cloned into NdeI and XhoI restriction sites | This study |
| pET21a::gtfU | pET21a containing gtfU gene cloned into NdeI and HindIII restriction sites; served as control | This study |
| pBBR1MCS | Broad-host-range vector; low copy; Cmr | 22 |
| pBBR1MCS-5 | Broad-host-range vector; low copy; Gmr | 22 |
| pBBR1MCS::Ptaq::His-dlhR | pBBR1MCS containing the His6 tag-dlhR fragment amplified by T7 promoter/T7 terminator primer from pET21a::dlhR | This study |
| pBBR1MCS::Ptaq::His-qsdR1 | pBBR1MCS containing the His6 tag-qsdR1 fragment amplified by T7 promoter/T7 terminator primer from pET21a::qsdR1 | This study |