Table 2.
Possible and verified QQ loci identified in the NGR234 genome
| ORFa | Possible and/or annotated function | Conserved HxHx∼DH motif | Size of protein (no. of amino acids) | Identity/similarity (%) to published QQ proteinb |
|---|---|---|---|---|
| NGR_c10650 | Metallo-β-lactamase family protein | + | 556 | |
| NGR_b16870 | Putative metallo-β-lactamase family protein QsdR1 | + | 321 | Aii2 (24/39) |
| NGR_b15850 | Metallo-β-lactamase family protein | + | 312 | AhlK (34/44) |
| NGR_b12260 | Metallo-β-lactamase family protein | + | 277 | |
| NGR_c22830 | Putative β-lactamase family protein | + | 214 | |
| NGR_c27960 | Metal-dependent hydrolase | − | 280 | |
| NGR_c05660 | Metallo-β-lactamase family protein | + | 304 | |
| NGR_c03760 | Metallo-β-lactamase family protein | + | 383 | Aii2 (27/44) |
| NGR_c05950 | Putative β-lactamase family protein | + | 254 | AhlD (30/47) |
| NGR_c16020 | Putative β-lactamase family protein QsdR2 | + | 279 | |
| NGR_c00430 | Putative metal-dependent hydrolase | − | 174 | |
| NGR_c08460 | Metal-dependent phosphohydrolase | − | 219 | |
| NGR_c06480 | Metal-dependent hydrolase | + | 289 | QlcA (27/49) |
| NGR_c32270 | Putative β-lactamase family protein | + | 336 | |
| NGR_c17430 | Metallo-β-lactamase family protein | + | 306 | |
| NGR_c10940 | Metal-dependent hydrolase | + | 235 | |
| NGR_b22150 | Dienelactone hydrolase-like protein DlhR | − | 358 | |
| NGR_c03800 | Dienelactone hydrolase-like protein | − | 292 | |
| NGR_c23150c | Acetaldehyde dehydrogenase AldR | − | 503 | |
| NGR_c35560 | Predicted NUDIX hydrolase (putative) | − | 346 | |
| NGR_c35570 | Histidine triad protein, hitR-hydR | − | 202 |
ORF identification numbers for NGR234 were derived from the GenBank entries U00090, CP000874, and CP001389. Genes/ORFs in bold were verified by functional searches in this work.
Identity and similarity values were obtained using the NCBI database. Only identities and similarities to the best hit are given: Aii2 from an uncultured Bacillus sp. (accession no. CAD44268), AhlK from Klebsiella pneumonia (accession no. AAO47340), AhlD from Arthrobacter sp. strain IBN110 (accession no. AAP57766), and QlcA from uncultured Acidobacteria, cosmid p2H8 (accession no. ABV58973).
The gene/ORF could be identified only by functional searches and was not found in the initial sequence-based searches.