Fig. 1.

Deletion in fhbA or supplementation of nitric oxide (NO) promotes A. nidulans sexual development. (A) Micrographs showing Hülle cells produced by ΔfhbA 5 days after inoculation on solid GMM (right), while no Hülle cells were detected in cultures of the isogenic control strain (left). wt, wild type. (B) Quantification of cleistothecia produced by the wild type and the fhb mutants with and without NO supplementation (core = 16-mm diameter). (C) Micrographs of the wild type and the fhb mutants with and without nitric oxide (NO) supplementation (35×). Cultures were sprayed with 70% ethanol to facilitate the visualization of cleistothecia.