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TCel thermostability. TCel enzymes (1.16 ± 0.156 μg protein/assay) were incubated at 90°C in phosphate-citrate buffer for the indicated number of hours, a sample was drawn from the master mix, and cellulase (CMC) or β-glucosidase (pNPG) activity was determined. The amount of residual activity is shown. With the exception of TCel3, all cellulases retained >80% of activity after 120 h at 90°C.
