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. 2011 Aug;77(15):5199–5206. doi: 10.1128/AEM.00199-11

Fig. 3.

Fig. 3.

(A to F) Capillary zone electrophoresis of breakdown products of APTS-labeled cellopentaose incubated with TCel1 (A), TCel2 (B), TCel3 (C), TCel4 (D), TCel5 (E), or TCel6 (F). (G) CZE retention times of purified monomer (DP1), dimer (DP2), trimer (DP3), tetramer (DP4), and the substrate cellopentaose (DP5). (H) Predicted cleavage patterns between DP2, DP3, and DP4. Assay conditions were as follows: substrate, APTS-cellopentaose; buffer, 50 mM sodium phosphate-50 mM citrate, incubation at 95°C and pH 6. CZE retention times vary slightly among electrophoresis runs. Since we used a defined substrate, retention time variations were not corrected.