Fig. 3.

Effects of bauR and γ-glutamylation on expression profiles of the bauA and pauA3 promoters. The specific activities of β-galactosidase expressed from pBAU1 for the bauA promoter or pPAU3 for the pauA3 promoter were measured from the host strains PAO1 (□), the bauR mutant (▩), and the M7 mutant (■) devoid of all seven pauA genes. The cells were grown in glutamate (Glu) minimal medium in the absence of presence of indicated supplements: diaminopropane (DAP), spermidine (Spd), and β-alanine (β-Ala).